Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Acceptor: 665nm. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 04. Integration delay. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. 7. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Manager: Yinghua Chen, PhD Contact Email: yinghua. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. 0% and ± 0. Emission. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Stat Fax 4700 Microstrip Reader. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 5. The membranes were. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Fig. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. StakMax. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. Crowder, Ziqian Zeng, Alissa N. 00. 4) was followed according to Vanacker et al. Details. 1 mL of culture media with 10% FBS. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Nitric Oxide Assay. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. . When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Dissolve 13. Molecular Devices SpectraMax Gemini XS Microplate Reader. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. 30. Streamline GLP/GMP compliance. Rate of fibrinolysis. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 30. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. The metals were preincubated with A for 5 min when added before heme. control. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). SpectraMax Multi-Mode Microplate Readers. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Calculating % of total mediator release in RBL assay. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. Details. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Pharmacokinetic and pharmacodynamic analysis. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Or scan up to 384 wells at any time to determine the best assay conditions. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. Filterless readers provide consistent results with steady temperature regulation. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. Samples with OD values of less than 0. The plates were incubated for 4 additional hours at 37°C. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. , Oxon, UK). 2. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. These robust readers have been placed in labs from Antarctica to the. SpectraMax 384 Plus. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. (1988) and recorded at 412 nm. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. This test was performed in duplicates. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 0 1. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 6) and 0. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 10. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. using SpectraMax i3 (Molecular devices, San Jose, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Microplate Readers. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. Data were expressed as the mean percent viable cell vs. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. The reaction mixture was incubated for 5 min. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. ) at 490 nm. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. 10× trypsin 0. TGFα release was calculated as described in the Results. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 2. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. Molecular Devices SpectraMax M5e Microplate Reader $26,500. Microplate Readers. The plate was covered and incubated for one hour at room temperature. Photometric Range: 0. Microplate Reader Shelf. The initial rate of hydrolysis was determined using the early linear region of. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 5. Anti-proteolytic activity. 2 - 3. 2. FACS analysis. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. These lab workhorses are used in drug discovery, research, bioassay validation, and. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. Endpoint, kinetic, spectrum. , Sunnyvale, CA, USA) at 595 nm. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 1% N-1-naphtylethylenediamine dihydrochloride, 2. edu Contact Phone: 216-368-8962. Molecular Devices Corporation 1311. Call 1-800-745-2710. The light features a slim profile to prevent. S. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The SPECTRAmax 340PC can measure. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 1. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. Driessen*,MaaikeA. 0. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. Acceptor filter: 665 nm. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 0 2. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. The slope of the linear portion of the kinetic curve generated from each well was used to. To measure the cell biomass (g/L),. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. 303 unable to cal dark current. Bioz Stars score: 86/100, based on 1 PubMed citations. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. Data were expressed as mean percentage of viable cells. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. Water – distilled water or equivalent. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. The first time you replace an OEM lamp you must use the N-34-MLA. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Alkaline phosphatase (ALP) activity. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. chen@case. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. 024). All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. control. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Each experiment was performed in duplicate and repeated at. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. SpectraMax QuickDrop Micro-Volume Spectrophotometer. Amino Acid Analysis. 0, 20 mmol L –1cal-cium chloride). Also for: Spectramax 190, Spectramax 340pc384, Versamax. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Lactate Dehydrogenase (LDH) Release. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. Table 1. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). It includes both a lamp and a new lamp holder. 312 gain calibration failed. The Catalina. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Detection limits. MCE has not independently confirmed the accuracy of these methods. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Products : SpectraMax Plus 384 reader,. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. Bertin Precellys Evolution Touch Homogenizer. Values were determined according to a standard curve (log/log curve-fit). WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. One plate carrier adapter is provided with the instrument. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. Includes: Computer Loaded with Softmax Pro 5. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. Subsequently, the mixture was heated at 100 °C for 10. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. The absorbances were being measured at 515 nm. 7. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 340 nm / 80 nm. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 5. PerkinElmer. One plate carrier adapter is provided with the instrument. The VersaMax and SpectraMax 190 read 96-well microplates. Examples: “Press carefully to engage,” “Do not press down. or microplate reader (e. As a common indicator of equine T cell activation, cytokine induction. 190-1000 nm, tunable in 1 nm increments. Donor filter: 620 nm. Wound healing assay. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). FlexStation® 3 Multi-Mode Microplate Reader. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. Percent Transmission Absorbance Competitor 0. 2 were considered negative for IgM antibody. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). 001/min at 425 nm. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. To. Statistical analysis. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. 304 signal level saturation. Assay setup for a 384-well low-volume plate. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 4; 100 m M NaCl, 0. Molecular Device M5e. This reader only allows high performance HTRF measurement when assays are run in. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. The loading equaled the mass of BSA per mass of particles. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The plates were incubated for 4 additional hours at 37°C. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). SpectraMax® Microplate Readers and SoftMax® Pro Software. 2. MMP‑1 inhibition assay. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. D. View the Molecular Devices website. Upgradeable modules are also available including western blot, cell imaging, and fast. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. 4. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The reaction mixture was incubated for 5 min. SpectraMax Multi-Mode Microplate Readers. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 3 0. Summary of Contents for Molecular Devices SPECTRAmax 340PC. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). 2. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 2 M DEEMM were mixed in a microtube,. albicans pre. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). See More Microplate Reader / Microtiter Plate Reader. reader (Molecular Devi ces). 00. Both readers feature an 8-channel read head for fast reads and. Details. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Operators can conveniently select up to 6 wavelengths at a time for. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 313 reference gain check fail. No power cord or accessories included. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. , Sunnyvale, CA). SPECTRAMAX 340. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. Introduction. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Stat Fax 4700 Microstrip Reader. Immunofluorescence and Aggregation Analysis. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Molecular Devices SpectraMax M5e Microplate Reader. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Glutathione reductase (GR) activity was measured, according to Smith et al. 25–0. (Shiga, Japan) and kept for 30 min in the dark. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Oxidative stress induction by H 2 O. Cell culture 3T3 fibroblasts and RAW 264. , Sunnyvale, CA). 2. SPECTRAmax® 190. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). 85%. (1993). TDP-43 stress granular cells were treated with 70% ice-cold. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. plates can be used in the SPECTRAmax PLUS 384. TGFα release was calculated as described in the Results. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 3 mm. Italic and boldface type are used for emphasis. Molecular Devices Spectramax 340PC instructions. , Sunnyvale, CA). 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Description 1. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. SoftMax Pro. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. reader (Molecular Devi ces).